Horak, I. R.     Adaptor protein Ruk/CIN85 modulates resistance to doxorubicin of murine 4T1 breast cancer cells / I. R. Horak, D. S. Gerashchenko, L. B. Drobot // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 3. - P94-100 MeSH-головна: МОЛОЧНОЙ ЖЕЛЕЗЫ ЖИВОТНЫХ НОВООБРАЗОВАНИЯ -- MAMMARY NEOPLASMS, ANIMAL АДАПТЕРНЫЕ БЕЛКИ ВЕЗИКУЛЯРНОГО ТРАНСПОРТА -- ADAPTOR PROTEINS, VESICULAR TRANSPORT ЛЕКАРСТВЕННАЯ УСТОЙЧИВОСТЬ -- DRUG RESISTANCE Анотація: The acquisition of chemoresistance in the course of tumor progression includes activation of membrane ABC transporters, detoxification enzymes, cell cycle deceleration and activation of specific signaling pathways such as Akt/mTOR, MAPK, NF-κB. Adaptor proteins play an essential role in the assembly of supramolecular signaling complexes, maintaining and directing the intracellular signaling. One of such proteins, called Ruk/CIN85, is strongly associated with malignant transformation and metastasis. In present study we investigated the Ruk/CIN85 effect of up/down-regulation on the transforming potential and doxorubicin resistance of highly aggressive mouse breast adenocarcinoma 4T1 cells. It was demonstrated that 4T1 cells overexpressing Ruk/CIN85 possessed increased resistance to doxorubicin (in the range of concentrations 0.1–10.0 µM) while knockdown cells were the most sensitive. Also, high levels of Ruk/CIN85 in 4T1 cells positively correlated with their ability to form colonies in semi-solid agar. Ruk/CIN85-overexpressing cells formed four times more colonies in comparison with Ruk/CIN85 nockdown cells, the growth of which revealed higher resistance to doxorubicin action Дод.точки доступу: Gerashchenko, D. S. Drobot, L. B. Вільних прим. немає