Kinetics of interaction between polyreactive immunoglobulins and antigen / S. A. Bobrovnik [et al.] // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 5. - P15-22 MeSH-головна: ИММУНОГЛОБУЛИНЫ -- IMMUNOGLOBULINS (метаболизм) АНТИГЕНЫ -- ANTIGENS (метаболизм) Анотація: A number of experimental kinetics curves of mice polyreactive immunoglobulins (PRIGs) binding to ovalbumin immobilized on immunologic plates were obtained at different temperatures. Analysis of these curves allowed us to conclude that the model of PRIGs interaction with antigens proposed by us earlier and consisted on PRIGs activation (i.e. exposition of hydrophobic patches on PRIGs surface) and either sequential binding to antigen or inactivation was is in a good agreement with the experimental data obtained in this study. We have designed a method of the rate constants evaluation from experimental binding curves. It was found that the rate constant of the activated PRIGs binding to immobilized antigen did not depend on temperature. The rate constant of PRIGs activation occurred to be depend on temperature more strongly than the rate constant of PRIGs inactivation. We have concluded from the acquired dependences that at 37°С the number of activated PRIGs was 15 times higher than that at 0°С Дод.точки доступу: Bobrovnik, S. A. Ogloblya, O. V. Demchenko, M. O. Komisarenko, S. V. Вільних прим. немає